HLA-A+B+C+E monoclonal antibody
Details
Concentration
1 mg/ml
Clone
TP25.99SF
French translation
anticorps
Antigen
HLA-A+B+C+E
Applications
FC, WB, IHC(F)
Purity
> 95% (by SDS-PAGE)
Species reactivity
Human (Homo sapiens)
Format/conjugation
purified; unconjugated
Clonality
Monoclonal Antibody (Mab)
Research area
MHC (Major Histocompatibility Complex) and Related Antigens (Human)
Long name
Mouse Monoclonal Antibody against Human HLA-A+B+C+E, Clone: TP25.99SF
Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Purification method
Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
Specificity
The mouse monoclonal antibody TP25.99SF recognizes HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other class I antigens.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Shipping and storage
Mouse Monoclonal Antibody against Human HLA-A+B+C+E, Clone: TP25.99SF is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
Background
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.
References
*Tanabe M, Sekimata M, Ferrone S, Takiguchi M: Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain. J Immunol. 1992 May 15;148(10):3202-9., *Blaschitz A, Hutter H, Leitner V, Pilz S, Wintersteiger R, Dohr G, Sedlmayr P: Reaction patterns of monoclonal antibodies to HLA-G in human tissues and on cell lines: a comparative study. Hum Immunol. 2000 Nov;61(11):1074-85., *Paul P, Rouas-Freiss N, Moreau P, Cabestre FA, Menier C, Khalil-Daher I, Pangault C, Onno M, Fauchet R, Martinez-Laso J, Morales P, Villena AA, Giacomini P, Natali PG, Frumento G, Ferrara GB, McMaster M, Fisher S, Schust D, Ferrone S, Dausset J, Geraghty D, Carosella ED: HLA-G, -E, -F preworkshop: tools and protocols for analysis of non-classical class I genes transcription and protein expression. Hum Immunol. 2000 Nov;61(11):1177-95.