FC, WB, IHC(F)
> 95% (by SDS-PAGE)
Human (Homo sapiens)
Monoclonal Antibody (Mab)
MHC (Major Histocompatibility Complex) and Related Antigens (Human)
Mouse Monoclonal Antibody against Human HLA-A+B+C+E, Clone: TP25.99SF
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Purified from hybridoma culture supernatant by protein-A affinity chromatography.
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
The mouse monoclonal antibody TP25.99SF recognizes HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other class I antigens.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Mouse Monoclonal Antibody against Human HLA-A+B+C+E, Clone: TP25.99SF is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.
*Tanabe M, Sekimata M, Ferrone S, Takiguchi M: Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain. J Immunol. 1992 May 15;148(10):3202-9., *Blaschitz A, Hutter H, Leitner V, Pilz S, Wintersteiger R, Dohr G, Sedlmayr P: Reaction patterns of monoclonal antibodies to HLA-G in human tissues and on cell lines: a comparative study. Hum Immunol. 2000 Nov;61(11):1074-85., *Paul P, Rouas-Freiss N, Moreau P, Cabestre FA, Menier C, Khalil-Daher I, Pangault C, Onno M, Fauchet R, Martinez-Laso J, Morales P, Villena AA, Giacomini P, Natali PG, Frumento G, Ferrara GB, McMaster M, Fisher S, Schust D, Ferrone S, Dausset J, Geraghty D, Carosella ED: HLA-G, -E, -F preworkshop: tools and protocols for analysis of non-classical class I genes transcription and protein expression. Hum Immunol. 2000 Nov;61(11):1177-95.